Protein-Protein Interactions Using Mass Spectrometry Coupled Techniques and Cellulose-Bound Peptide Arrays: Mapping, Affinity Purification, Quality of Protein Interaction Data and Peptide Synthesis Strategies

The present book, Protein-Protein Interactions Using Mass Spectrometry Coupled Techniques and Cellulose-Bound Peptide Arrays: Mapping, Affinity Purification, Quality of Protein Interaction Data, and Peptide Synthesis Strategies is a definitive exploration into the realm of protein interaction studies, leveraging cutting-edge methodologies to uncover intricate molecular relationships. This book begins with a comprehensive introduction, setting the stage for understanding interaction mapping and protein complex affinity purification techniques. It delves into advanced methods such as FLAG tag immunopurification and phosphopeptide purification, highlighting their efficacy in studying protein interactions without the constraints of traditional gel-based approaches. Central to the text is the application of mass spectrometry in elucidating protein interaction data quality, including its role in enzyme-protein interaction registries and proximity labeling studies. The integration of cellulose-bound peptide arrays provides a novel approach to scale protein-protein interaction analysis, discussing synthesis strategies and assay methodologies. From crosslinking mass spectrometry (XL-MS) to exploring host-pathogen interactions, each chapter presents breakthrough insights into the dynamic landscape of proteomics research. This book serves as an indispensable resource for researchers and practitioners in biochemistry, molecular biology, and biotechnology, offering a profound synthesis of theoretical foundations and practical applications in deciphering protein-protein interactions at a molecular scale.